Activity Number
260
Not Editable
Overview and Learning Objectives
Assessment
Central Concepts
Textbook References
Benchmarks and Standards
Activity Credits
Requirements

Template-Based Synthesis: PCR

Interactive, scaffolded model

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Overview and Learning Objectives

Students learn that it is difficult, if not practically impossible, for organisms to create a polymer without some guiding template molecule. DNA is then discussed as an example and the basics of DNA replication are explored. Finally, students learn how the natural mechanism of DNA replication can be used to amplify small amounts of DNA using the Polymerase Chain Reaction (PCR) procedure.

Students will be able to:

  • demonstrate how using a template is an efficient way to create a molecule of specific sequence;
  • discuss the basic mechanism of how DNA replicates;
  • apply their knowledge of DNA replication to the Polymerase Chain Reaction procedure.

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Assessment

http://www.concord.org/~barbara/workbench_web/pdf/pcr_assessment_fin5.pdf

http://www.concord.org/~barbara/workbench_web/pdf/pcr_assessment_RUBRIC5.pdf

You can also use Molecular Workbench to enter and send your assessment:

http://www.concord.org/~barbara/workbench_web/models/jnlps/PCR_assess1.jnlp

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Central Concepts

Key Concept:

Using DNA's capacity to make many copies of itself, the laboratory procedure Polymerase Chain Reaction (PCR) replicates DNA samples for forensics and drug development.

Additional Related Concepts

Biology

  • Genetics

Molecular Biology

  • Coding
  • DNA
  • Nucleic Acid
  • Template-based synthesis

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Textbook References

  • Biology (Prentice-Hall) New York Edition - Chapter 12: DNA and RNA
  • Biology: Concepts and Connections (Pearson) 5th Edition - Chapter 10: Molecular Biology of the Gene
  • Biology: Concepts and Connections (Pearson) 5th Edition - Chapter 11: The Control of Gene Expression
  • Biology: Exploring Life - Chapter 9: The Cellular Basis of Inheritance
  • Biology: The Dynamics of Life - Chapter 13: Genetic Technology
  • BSCS Blue (8th Edition) - Advances in Molecular Genetics
  • Web of Life - Chapter 7: DNA, Genes and Chromosomes

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Benchmarks and Standards

AAAS

  • COMMON THEMES: Systems - The successful operation of a designed system usually involves feedback (Full Text of Standard)

  • THE LIVING ENVIRONMENT: CELLS - The genetic information encoded in DNA molecules provides instructions for assembling protein molecules (Full Text of Standard)

NSES

  • Science as Inquiry: 3 Use technology and mathematics to improve investigations and communications (Full Text of Standard)

  • Science as Inquiry: 4 Formulate and revise scientific explanations and models (Full Text of Standard)

  • Science as Inquiry: 5 Recognize and analyze alternative explanations and models (Full Text of Standard)

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Activity Credits

Created by CC Project: Molecular Workbench using Flash and Molecular Workbench + Pedagogica

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Requirements

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NSF Logo
These materials are based upon work supported
by the National Science Foundation under grant numbers
9980620, ESI-0242701, EIA-0219345, DUE-0402553, and 0628181.

Any opinions, findings, and conclusions or recommendations expressed in this
material are those of the author(s) and do not necessarily reflect
the views of the National Science Foundation.