Activity Number 199
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	Overview and Learning Objectives
	Assessment
	Classroom Practice
	Central Concepts
	Textbook References
	Benchmarks and Standards
	Extensions and Connections
	Activity Credits
	Requirements

Electrophoresis, with SDS Page (an 7-page activity for biotechnology)

Interactive, scaffolded model

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Java 1.4+ - Java 1.4+ is available for Windows, Linux, and Mac OS X 10.3 and greater.

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	Overview and Learning Objectives Electrophoresis is a technique used to separate proteins and DNA fragments. Here students explore the role electrostatic forces and mobility of particles play in the process. Students review diffusion and then proceed to explore several types of electrophoresis. Students will be able to: separate proteins and DNA fragments by size, charge, and mass; generate and analyze separation graphs. return to top



	Assessment TEST http://www.concord.org/~barbara/workbench_web/pdf/electro_assess_v5.pdf RUBRIC http://www.concord.org/~barbara/workbench_web/pdf/electroph_rubric_v6.pdf return to top



	Classroom Practice http://gslc.genetics.utah.edu/units/activities/electrophoresis/ return to top



	Central Concepts Key Concept: Electrophoresis is a technique used to separate proteins and DNA fragments. It uses two factors in the separation: the mobility of different sized molecules in a gel matrix; electrostatic forces that can be applied to charged molecules. Additional Related Concepts Molecular Biology Protein structure Physics/Chemistry Electromagnetic force return to top



	Textbook References Biology: Concepts and Connections (Pearson) 5th Edition - Chapter 12: DNA Technology and the Human Genome Cell Biology (Pollard and Earnshaw) Saunders 2002 - Chapter Five: Research Strategies Web of Life - Chapter 8: Protein Synthesis return to top



	Benchmarks and Standards NSES Science as Inquiry: 3 Use technology and mathematics to improve investigations and communications (Full Text of Standard) return to top



	Extensions and Connections See the animation: http://courses.cm.utexas.edu/jrobertus/ch339k/overheads-1/03_SDS_gel_electro.html Sickle Cell Story. Because Sickle Cell Hemoglobin (HbS) has a difference in charge, it moves more slowly through an alkaline solution with a current than normal hemoglobin. Other sickle cell disorders move at their own speeds through the solution and researchers can diagnosis the different ailments. return to top



	Activity Credits Created by CC Project: CCATOMS and CC Project: Molecular Logic using Molecular Workbench + Pedagogica return to top



	Requirements Java 1.4+ - Java 1.4+ is available for Windows, Linux, and Mac OS X 10.3 and greater. return to top

These materials are based upon work supported
by the National Science Foundation under grant numbers
9980620, ESI-0242701, EIA-0219345, DUE-0402553, and 0628181.

Any opinions, findings, and conclusions or recommendations expressed in this
material are those of the author(s) and do not necessarily reflect
the views of the National Science Foundation.

Electrophoresis, with SDS Page (an 7-page activity for biotechnology)

Overview and Learning Objectives

Assessment

Classroom Practice

Central Concepts

Additional Related Concepts

Textbook References

Benchmarks and Standards

Extensions and Connections

Activity Credits

Requirements