Activity Number
199
Not Editable
Overview and Learning Objectives
Assessment
Classroom Practice
Central Concepts
Textbook References
Benchmarks and Standards
Extensions and Connections
Activity Credits
Requirements

Electrophoresis, with SDS Page (an 7-page activity for biotechnology)

Interactive, scaffolded model

Activity Screenshot

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This Activity Requires:

  • Java 1.4+ - Java 1.4+ is available for Windows, Linux, and Mac OS X 10.3 and greater.

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Overview and Learning Objectives

Electrophoresis is a technique used to separate proteins and DNA fragments. Here students explore the role electrostatic forces and mobility of particles play in the process. Students review diffusion and then proceed to explore several types of electrophoresis.

Students will be able to:

  • separate proteins and DNA fragments by size, charge, and mass;
  • generate and analyze separation graphs.

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Assessment

TEST

http://www.concord.org/~barbara/workbench_web/pdf/electro_assess_v5.pdf

RUBRIC http://www.concord.org/~barbara/workbench_web/pdf/electroph_rubric_v6.pdf

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Classroom Practice

http://gslc.genetics.utah.edu/units/activities/electrophoresis/

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Central Concepts

Key Concept:

Electrophoresis is a technique used to separate proteins and DNA fragments.

It uses two factors in the separation:

  • the mobility of different sized molecules in a gel matrix;
  • electrostatic forces that can be applied to charged molecules.

Additional Related Concepts

Molecular Biology

  • Protein structure

Physics/Chemistry

  • Electromagnetic force

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Textbook References

  • Biology: Concepts and Connections (Pearson) 5th Edition - Chapter 12: DNA Technology and the Human Genome
  • Cell Biology (Pollard and Earnshaw) Saunders 2002 - Chapter Five: Research Strategies
  • Web of Life - Chapter 8: Protein Synthesis

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Benchmarks and Standards

NSES

  • Science as Inquiry: 3 Use technology and mathematics to improve investigations and communications (Full Text of Standard)

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Extensions and Connections

  1. See the animation:

http://courses.cm.utexas.edu/jrobertus/ch339k/overheads-1/03_SDS_gel_electro.html

  1. Sickle Cell Story. Because Sickle Cell Hemoglobin (HbS) has a difference in charge, it moves more slowly through an alkaline solution with a current than normal hemoglobin. Other sickle cell disorders move at their own speeds through the solution and researchers can diagnosis the different ailments.

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Activity Credits

Created by CC Project: CCATOMS and CC Project: Molecular Logic using Molecular Workbench + Pedagogica

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Requirements

  • Java 1.4+ - Java 1.4+ is available for Windows, Linux, and Mac OS X 10.3 and greater.

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NSF Logo
These materials are based upon work supported
by the National Science Foundation under grant numbers
9980620, ESI-0242701, EIA-0219345, DUE-0402553, and 0628181.

Any opinions, findings, and conclusions or recommendations expressed in this
material are those of the author(s) and do not necessarily reflect
the views of the National Science Foundation.